3. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Using cross-feeding bioassay, a functional LuxI/LuxR-type acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) system. Fifteen different automated tests are provided: • Background noise • Background spike • Lower limit of detection • Crosstalk • Linearity • Relative luminescence units (RLU) • Kinetic noise (low signal)0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M3, M4, M5, and M5 e. Cellular viability was assessed by using resazurin at 1. 304 signal level saturation. Note: In this user guide, SpectraMax ® refers to several SpectraMax . Recertification is recommended every two years. Lysosomal proteolytic activity. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf, Switzerland). ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). . Page: 1/6. The aim of the present study was to investigate the preventive effects of. There are multiple vendors. Then, the fluorescence intensity was quantified by using a spectrophotometer (Spectramax M4, MolecularDevices, San Jose, CA, USA) at λ ex: 579 nm and λ em: 599 nm. Turn the instrument around so that the back of the instrument is. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Test samples were measured according to the manufacturer’s instructions using a spectrophotometer SpectraMax M4 (Molecular Devices, Sunnyvale, CA). 5 mg mL −1 with 0. With optimized reagents, option to operate the. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. Then, MTT (10 μl of 5 mg/ml) was added to each well and further incubated for 4 h. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Data was calculated subtracting OD440 recorded with the final OD600 values. The available phosphorus in the soil was leached by double-acid extraction (0. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. org) with optimized instrument settings (Table 1) were used to generate and analyze data. FlexStation™ II. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. After incubation, the absorbance value of all samples was measured at 517 nm on a Spectramax M4. SpectraMax® iD5. FlexStation™ I. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. MTT values at day 3 were normalized to day 0. The medium was then removed from the wells, and 100 µl of DMSO was added to solubilize the formazan crystals. 2. StakMax Handling Stacker Base Unit. The plate reader. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. The fluorescent signal at an excitation wavelength of 530 nm and emission wavelength of 580 nm was measured by Spectramax M4, USA. If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the. . Moving a SpectraMax Multi-Mode Microplate Reader. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 4. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. 2. The UV‐Vis absorption spectra and cell viability were measured by a multi‐mode microplate reader (SpectraMax® M4, Molecular Devices LLC) using quartz cuvettes with 1‐cm path length (Starna Scientific Ltd. The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. SpectraMax® Aquamax. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. siRNA-mediated knockdown of KLF2 in RAW 264. Italic and boldface type are used for emphasis. 6 mm). 73. To investigate the pharmacokinetics of HSA–CBT-NPs, 10 male Sprague-Dawley rats (mean±SD=250±20A SpectraMax M4 microplate reader was purchased from MeiGu Molecular Co. Introduction. Table of Contents. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Cells were exposed to drug solutions with. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. 313 reference gain check fail. C. . . The absorbance of formazan was detected at 540-nm wavelength with a reference wavelength at 650 nm for background using a SpectraMax M4 plate reader (Molecular Devices). 6 reviews. 2. . Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. 3, La Jolla, CA, USA). Finally, the optical density was evaluated using SpectraMax M4 plate reader (Molecular Devices, San Jose, CA, USA) at 450 nm. . 1. 314 low lamp level warning. Control AM were incubated in the same buffer for 1 h at 37 °C. After 3 times washing, cells were added into 96-well plate and were examined under a fluorescence microplate reader (Excitation, 345 nm; Emission 495 nm) (SpectraMax M4 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA). Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. Figure 1. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 2. Home; Lighting; Work lights; User manual. The Multi-mode readers are top and bottom read. /525 em. Background-subtracted data values (mean +/- SD) are graphed. neoformans was cultured from glycerol stock in 5 mL of YM broth at 25°C for 72 hours at 200 rpm. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. Plot the fluorescence intensity versus the cell seeding number. After aerosol collection was completed, the impactor plates were rinsed with 0. For heme titration experiments a constant ATP concentration of 1 mM was used. Supplier: Molecular Devices. SpectraMax M4 Multi-Mode Microplate Reader. . The innovative and flexible design features. NCI-60 Human Tumor Cell Line Screen. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader 5 B > 4 4 5 B 5 : F 8 8 > 3 ; > I 5 = 8 5. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. Detection modalities are shown in Table 1-1. book Page 1 Wednesday, July 7, 2010 2:09 PM SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. All experiments. Plot the fluorescence intensity versus the cell seeding number. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. 1. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. 2. Interpolated concentrations of native CRP in human serum and plasma samples. . Dettmer4, Wayne A. The luciferase values were determined by normalization to Renilla values (as a control) to correct for variation in transfection efficiencies. Dumont3, Matthias S. NCI-60 cell panel screening was performed by the NCI/National Institutes of Health developmental therapeutics program (Bethesda, US). The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Background-subtracted data values (mean +/- SD) are graphed. , UK) or SpectraMax M4 by Molecular Device. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. 5. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. Spectramax M4 Multi mode Microplate Detection System: Benchpro 4100 instrument card processing station: Muse Cell Analyzer: Veriti 96w Thermal Cycler: Small Animal Anaesthesia System: HPLCs (Analytical & Preparative) ACQUITY UPLC H-Class Bio: LC-MS/MS Q-tof 6540: Gas Chromatograph: GC-2014: Automatic Digital Polarimeter: FT-IR. TSQ was dissolved in Lock’s buffer (pH 7. The IC50 curves were calculated using variable slope for log (inhibitor) vs. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . Choose from a three- (M3), four- (M4), orThe integration time suggested is 5-10 sec. Luminescence was immediately measured by a SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA) after mixing the fecal supernatant and Hgb standard with the same amount of luminol reagent. On day 2, in the morning weight the mice. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. The BD plate dimensions are 5. D 47 7. book Page 1 Wednesday, July 7, 2010 2:09 PMTo quantify the cell density of bacterial cells, a SpectraMax M4 Microplate Reader was used to take measurements of the optical density at 600 nm. Operated from(A) ROS levels and (B) NAD+/NADH ratios in rotenone-pretreated young HDFs. Page 17 Chapter 2: Set Up the StakMax Microplate Handler Microplate Handler Mounted Baseplate Connection This procedure provides instructions to connect the microplate handler to the following instruments: Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax. SpectraMax Microplate Readers. Parameter SpectraMax iD5 SpectraMax i3x SpectraMax. Example: [Drawer]. Large Molecule Bioanalysis. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 39. Results were expressed as the mean percentage of CV compared with NC group. 1 Figure from 528 Publications Molecular Devices LLC. The absorbance was measured spectrophotometrically using a microplate reader (SpectraMax M4, Molecular Devices, CA, USA) at 570 nm. 4) and cells were stained with 150 nM TSQ for 1 min. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. normalized response (Graphpad Prism 8. Background-subtracted data values (mean +/- SD) are graphed. Molecular Devices. • SpectraMax® Paradigm (multi-mode) • SpectraMax® M5e / M5 (multi-mode) • SpectraMax® M4 / M3 (multi-mode) • SpectraMax® M2e / M2 (dual mode) • Gemini ™ EM and Gemini ™ XPS (fluorescence) • SpectraMax® L • SpectraMax® Plus384 (serial number # -MNR-xxxxx)The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 2. RFU was plotted versus time to derive the rate of formation of the fluorophore. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. Table 2. /600 em. The absorbance was recorded using Spectramax M4 (Molecular Devices, USA) at 440 nm. Other technologies such as imaging. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. 305 reference level saturation. > spectramax m4 microplate reader. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. TSQ was dissolved in Lock’s buffer (pH 7. SpectraMax M4 Multi-Mode Microplate Reader. Bioz Stars score: 98/100, based on 1 PubMed citations. The optical density was measured using a microplate reader (Spectramax M4, Molecular Devices, San Jose, CA, USA) at 570 nm. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. For more detailed information and technical support of Molecular Devices instruments or software,Luciferase Detection Instrumentation Guide. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. We therefore gathered salient information about many of the. . Note: In this user guide, SpectraMax ® refers to several SpectraMax . book Page 1 Wednesday, July 7, 2010 2:09 PMLuciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 5. All configurations. 1 & 7. Table 2. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. Results were normalized and expressed as a percentage of live cells. Background-subtracted data values (mean +/- SD) are graphed. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M4 Multi-Mode Microplate Reader. 3752 in as per SBS dimensions. SpectraMax ABS Plus SpectraTest ABS2 v1. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Environmental Chamber and Respiratory SimulatorsRhizoma drynariae, a traditional Chinese herb, is commonly used in treatment of bone healing in osteoporotic fractures. Background-subtracted data values (mean +/- SD) are graphed. DMSO was used to solubilize the formazan crystals and absorbance was measured at 570 nm (Spectramax M4, Molecular devices, USA). Standard curves for quantifying samples were generated by using serial dilutions of BSA from 0 to 0. (Fair Lawn, NJ, USA). High-performance liquid chromatography (HPLC) chromatograms were performed on Agilent Infinity 1260 with a C18 column (100 Å, 30 × 4. Simulated Exposure SystemOptical density readings were obtained at various timepoints from 0 to 128 hours following inoculation using a SpectraMax M4 spectrophotometer (Molecular Devices, San Jose, CA). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax® L (software version 5. Samples (2 mg) were mixed with potassium bromide in a 1:100 ratio before the analysis. FD-4 concentrations in plasma were analyzed in duplicates using a spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485 nm and emission λ 535 nm. The intestinal barrier has been recognized as being of major relevance for a number of diseases, namely digestive diseases like inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and celiac disease (CD), but also extra-intestinal diseases, such as asthma, arthritis, and the metabolic syndrome [1–4]. book Page 1 Wednesday, July 7, 2010 2:09 PM. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. WARNING! The instrument weighs approximately 36 pounds (16. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Cet appareil ISM est confomre à la norme NMB-001 du Canada. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). D 1 1. Dumont3, Matthias S. Qualify the luminescence performance of the SpectraMax . User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. 05, ** p <. 0 compensates for most changes in environmental temperature or pressure. The optical systems Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. 5. The chamber contains springs that position the cuvette in the proper alignment for a read. A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. The SpectraMax M5e reader offers the additional benefit of being certified . Compare plate readers7 Chapter 1: Installation and Setup SoftMax Pro Microplate Data Acquisition and Analysis Software controls Molecular Devices spectrophotometers, absorbance, luminescence, and fluorescence microplate readers, and the SpectraMax Paradigm Multi-Mode Detection Platform. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. DNA standards. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. Detection modalities are shown in Table 1-1. After 48 h, the optical density (OD) was measured at 450 nm using the microplate reader (SpectraMax M4). The MTT reagent was incubated with cells for 4 h. The drawing shows a standard microplate of 15 mm height covered with a lid. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. ® reader to the computer. Pierce™ BCA Protein Assay Kit was purchased from Thermo Fisher Scientific Inc. 05 mg mL −1. The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. instruments you must first insert an adapter plate. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 1% CF3COOH) = 5/95 (v/v) to 95/5 (v/v); flow rate:The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. com. Danaher Inc spectramax m series Spectramax M Series, supplied by Danaher Inc, used in various techniques. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The conjugate was also characterized using FTIR (FT/IR-4200, Jasco Inc. . The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Vol 4, Iss 22, Nov 20, 2014 . For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 3, La Jolla, CA, USA). Brand: Molecular. Colony formation assays were performed to measure cell proliferation. Members of the Sphingomonadales are renowned for their ability to degrade polycyclic aromatic hydrocarbons (PAHs). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. In vitro transcription (IVT) is an essential technique for RNA synthesis. Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). D 1 1. Note: In this user guide, all references to SpectraMax Multi-Mode. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR-FRET、蛍光偏光アッセイにおいても専用機に匹敵す. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. High tolerance for contaminants. 5 pM 384-well (50 µL) 6 pM 10 pM 1536-well (8 µL) 19 pM 25 pM 0200-7015 (LUM 384) Optimized Guaranteed* 96-well (200 µL) 1. Prepare 22 mg/mL of FD4, 16 mg/mL of rhodamine70, and 40 mg/mL of creatinine in sterile PBS. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). ZERO BIAS - scores, article reviews, protocol conditions and more7. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, AquaMax washer, SpectraMax Plus 384 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax L reader, SpectraMax M5/M5e. Large molecule bioanalysis, immunogenicity and PK testing with higher quality data, rapid turnaround and fewer surprises. SigmaPlot (Systat Software, Inc. 316 grating motor driver faulty. SpectraMax® L (software version 5. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. +1 250-415-0785. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 2. Highly selective for dsDNA over ssDNA or RNA. In the M2, M5 and Plus. 4. Hemoglobin release from erythrocyte lysis was detected by measuring the optical density (OD) of the supernatant at 412 nm using a microplate reader (SpectraMax M4 from Molecular Devices, or Tecan SPARK, from Tecan, Männedorf,. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. Choose from a three- (M3), four- (M4), orLuciferase Detection Instrumentation Guide. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Ltd. Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 72 MB. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1, FlexStation 3 reader, SpectraMax L reader, LMax II (Obsolete), SpectraMax M5/M5e reader. The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. Highly selective for dsDNA over ssDNA or RNA. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. STAKMAX. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Expand the ABS1 Plate Inserted or Expt#3 experiment and select the OptAlign&UltDrk Plate section. Where is the instrument serial number located? The serial number is on. 1. Description 1. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. 2. . The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. D 1 1. 光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 140 多种预存实验方法可以直接调用，可以进行数 据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功 能，可以方便的进行各种数据结果的展示。 完整的. Measurement of Mitochondrial Membrane Potential by JC-1. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. View specifications, prices, citations, reviews, and more. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. 116 SpectraMax M5 and M5e Multi-Mode. 2. org) with optimized instrument settings (Table 1) were used to generate and analyze data. Description 1. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. • Tel. 0 calibration logic, which needs only 5 minutes and a single sample per day rather than the 30 or more minutes needed by conventional analyzers. Description 1. overnight. 2. Choose from a three- (M3), four- (M4), orThe optical density was determined spectrophotometrically at 405 nm using a SpectraMax M4 microplate reader (Molecular Devices, Sunnyvale, CA). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. 7 cells. Plates were then read at 450 nm using a SpectraMax M4 device (Molecular Devices, Sunnyvale, CA). 383 inches whereas the maximum allowed is 5. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. of a 96 well-plate and cultured for 24h at 37 °C. Using. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. Fluorescence Microplate Reader Comparison Chart. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). , San Jose, CA) was. Images were taken using a fluorescence microscope (Nikon 80i, Japan). • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. Data are presented as mean values ± standard. 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. Cell viability was calculated using the following formulaThe luciferase signal was read in a plate reader (SpectraMax M4; Molecular Devices, Sunnyvale, CA). Interpolated concentrations of native PF4 in human serum, plasma and cell culture supernatant samples. . 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. ” The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Results and Discussion Small-molecule agonists have been identified for TLR7 [ 41 , 42 , 47 , 48 , 60 – 67 ], TLR8 [ 49 , 51 – 54 , 68 ], TLR4 [ 69 – 72 ] and TLR2 [ 73 , 74 ]. ® reader to the computer. VIEW DETAILS. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Western blot apparatus (BIO-RAD Laboratories). The biofilm mass was quantified through the measurement of optical density of the sample at 570 nm using a spectrophotometer (SpectraMax® M4 Multi-Mode Microplate Reader, Molecular Devices). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual.